Pengaruh Kinetin dan BAP terhadap Pertumbuhan dan Perkembangan Embrio Somatik Tanaman Sagu (Metroxylon sagu Rottb.)
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Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
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Effect of Kinetin and BAP to Growth and Development ofSomatic Embryos of Sago Palm (Metroxylon sagu Rottb.).Imron Riyadi. Somatic embryos induction in sago palm(Metroxylon sagu Rottb.) have succesfully developed. Kindsand concentration of plant growth regulators (PGR’s)influence to growth and development of somatic embryos.The research was conducted to determine the optimal concentrationof BAP and kinetin for proliferation, maturationand germination of sago palm somatic embryos. Cotyledonstageof somatic embryo derived from shoot tip culturescultured on Modified Murashige-Skoog (MMS) with halfstrengthmacro-salts and added with 30 g/l sucrose, 2 g/lgelrite, 1 g/l activated charcoal. pH of media was adjusted at5.6 before sterilized. The media were supplemented with0.1-2.0 mg/l BAP and 0.1-2.0 mg/l kinetin in combination with0.01 mg/l ABA each for supporting growth and development.The cultures was incubated at 26+1oC under a 12-hphotoperiod with lighting providing an intensity 20 μmolesphotons/m2/second for 11 weeks with replication 10 times.The results showed that the highest of somatic embryoproliferation was achieved in a culture medium with BAP at0.5 mg/l + 0.01 mg/l ABA with an expression rate of 94%, thebest maturation at 1.0 mg/l kinetin + 0.01 mg/l ABA with anexpression rate of 93.5% and the most germination at 2.0mg/l kinetin + 0.01 mg/l ABA with an expression rate of100%. Transfer of these germinants to gelled media withoutPGR’s led to the development of normal plantlets.
Keywords
BAP; kinetin; germination sago palm; somatic embryogenesis; maturation; Metroxylon sagu Rottb