Regenerasi Empat Genotipe Gandum (Triticum aestivum L.) Pasca Transformasi Melalui Agrobacterium tumefaciens

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dc.contributor en-US
dc.creator Apriana, Aniversari; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creator Sisharmini, Atmitri; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creator Fitriyani, Fitriyani; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creator Sustiprijatno, Sustiprijatno; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.date 2016-08-23
dc.date.accessioned 2019-10-09T09:40:27Z
dc.date.available 2019-10-09T09:40:27Z
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/4136
dc.identifier 10.21082/jbio.v10n1.2014.p18-25
dc.identifier.uri http://124.81.126.59/handle/123456789/7676
dc.description Genetic transformation ofwheat mediated by Agrobacterium tumefaciens has notbeen established yet. This research aimed to obtain themost responsive wheat genotype to be transformed, toselect the most effective combination of growth regulator forregenerating transforman calli, and to determine thetransformation efficiency. Transformation mediated by A.tumefaciens was performed on four genotypes of wheat,namely Combi, Fasan, Perdix, and Naxos-Wew.Transformed calli with green spots in selection media weretransferred to regeneration media containing 25 mg/lhygromycin, i.e. R1H25 and R2H25. The obtained plantletswere analyzed by PCR using specific primers for hygromycinphosphotransferase gene. The results showed that Fasanwas the most responsive genotype in callus formation(95.47%) and regeneration both in R1H25 (27%) and R2H25(28.6%) media. Fourteen plantlets were succesfullyacclimatized and PCR analysis showed that there were fourpositive transformants containing the hpt gene. The resultsare expected to provide information on the development oftransgenic wheat in Indonesia, specifically in the success ofcallus formation and regeneration of wheat aftertransformation using A. tumefaciens. en-US
dc.format application/pdf
dc.language eng
dc.publisher Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/4136/3451
dc.rights Copyright (c) 2016 Jurnal AgroBiogen en-US
dc.source Jurnal AgroBiogen; Vol 10, No 1 (2014): April; 18-25 en-US
dc.source 2549-1547
dc.source 1907-1094
dc.subject Wheat; transformation study; Agrobacterium. en-US
dc.title Regenerasi Empat Genotipe Gandum (Triticum aestivum L.) Pasca Transformasi Melalui Agrobacterium tumefaciens en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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