DIVERSITY AND CAPABILITY ANALYSES OF FERTILITY RESTORER GENES OF CYTOPLASMIC MALE STERILE RICE LINES USING SSR

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dc.contributor Indonesian Center for Rice Research en-US
dc.creator Widyastuti, Yuni; Indonesian Center for Rice Research
dc.creator Yunus, Muhamad; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development
dc.creator Purwoko, Bambang Sapta; Department. of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University
dc.creator Satoto, Satoto; Indonesian Center for Rice Research
dc.date 2018-01-30
dc.date.accessioned 2018-05-02T01:18:07Z
dc.date.available 2018-05-02T01:18:07Z
dc.date.issued 2018-01-30
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/7372
dc.identifier 10.21082/ijas.v18n2.2017.p43-50
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/49
dc.description Development of hybrid rice depends on the effectivity of cytoplasmic male sterility (CMS) and restorer (R) lines.  The molecular genetic approach is expected to help the breeder in identification of suitable parental lines to hybrid rice improvement. The study aimed to assess genetic relationship among three types of CMS systems (wild  abbortive/WA Kalinga and Gambiaca) as female parents and to identify diversity of genes controlling fertility restoration in rice. The study used nine F1 hybrids and F2 populations obtained from the hybridization of three different CMS lines (IR58025A-WA, IR80156A-Kalinga and IR80154A-Gambiaca) with three restorer lines (PK90, PK12 and BP11). Fifteen SSR markers were used to select genomic regions of chromosome 1 and 10 on which Rf3 and Rf4 genes located in the hybrids. The results showed that fertility restoration in CMS-WA and CMS-Gambiaca was governed by two independent and dominant genes (Rf3 and Rf4), while in CMS-Kalinga the fertility restoration was controlled by one single dominant gene. Biological processes occurred in the fertility restoration of the hybrids were the same based on the pollen and spikelet fertilities of F1 hybrids derived from three CMS and R lines, i.e. 76.1–78.3% and 69.1–76.6%, respectively. A restorer line PK12 had a higher capability in fertility restoration than PK90 and BP11. The SSR primers RM490 and RM258 were capable of identifying the Rf3 and Rf4 genes controlled fertility restoration in CMS-WA. The study supports the use of male sterile WA in rice hybridization.   en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Agency for Agricultural Research and Development en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/7372/6952
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/downloadSuppFile/7372/510
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/downloadSuppFile/7372/511
dc.rights Copyright (c) 2017 Indonesian Journal of Agricultural Science en-US
dc.rights http://creativecommons.org/licenses/by-sa/4.0 en-US
dc.source 2354-8509
dc.source 1411-982X
dc.source Indonesian Journal of Agricultural Science; Vol 18, No 2 (2017): December 2017; 43-50 en-US
dc.title DIVERSITY AND CAPABILITY ANALYSES OF FERTILITY RESTORER GENES OF CYTOPLASMIC MALE STERILE RICE LINES USING SSR en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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