Fermentative Gas Production of Different Feeds Collected During Wet and Dry Seasons when Incubated with Rumen Fluid from Timor Deer (Cervus timorensis)

Abstract
Description
An experiment was done to investigate rumen microbial fermentation of various feeds comonly given to Timor deer (Cervus timorensis) using the in vitro gas production technique. Seven forage feed sample (Sesbania grandiflora, Leucaena leucocephala, Glyricidia sepium, Zea mays, Ipomea aquatica, Pennisetum purpureum, and native grass) collected during dry and wet seasons and three concentrate feed samples (rice bran, copra meal and tofu waste) were dried and ground. The feed sample (200 g) was transferred into incubation syringe which was then added with incubation medium (mixture of rumen fluid and buffer solution). The rumen fluid was collected from 2 Timor deer using a trokar technique. Incubation was run for 72 h, and gas production was read at 2, 4, 6, 12, 24, 48, and 72 h of incubation. Data were fitted to an exponential equation generally used in similar in vitro gas production studies. Results of the study indicated that there was an effect of season on fermentation parameters (values of a, b or c) for the same feed, but this effect was not consistent from one feed to another. From data of total and cummulative gas productions, it was indicated that Zea mays in either dry or wet season showed the highest, while Leucaena leucocephala exhibited the lowest gas production compared to other feeds tested.
Keywords
Cervus Timorensis; Gas Poduction; Wet Season; Dry Season; Fermentation
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