Genotyping of Kappa-Casein Gene of Buffalo in Indonesian

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dc.contributor en-US
dc.creator Margawati, Endang T; Research Center for Biotechnology of Indonesian Institute of Sciences
dc.creator Volkandari, S D
dc.creator ., Indriawati
dc.creator Talib, Chalid 2017-03-01 2018-06-04T07:56:31Z 2018-06-04T07:56:31Z
dc.identifier 10.14334/Proc.Intsem.LPVT-2016-p.37-44
dc.description Casein (KCN) is a milk protein with four variants of alpha S1, alpha S2, beta and kappa which has different allel of each. Kappa Casein (CSN3) gene lies at exon 4 associating with fat and protein milk contens. Genetic variation of CSN3 gene influences quality and composition of milk. The aim of this research was to genotype the CSN3 gene of Bufalos from several places in Indonesia. Amount of 29 buffalo samples was used in this research, those consisted of 15 collected from Medan, 3 samples of Banyuwangi, 10 samples of Baluran and one sample of South-East Nusa Tenggara (NTT). A Polymerase Chain Reaction-Restiction Fragment Length Polymorphism (PCR-RFLP) method was used for CSN3 gene analysis. The CSN3 gene was amplified at 56°C of annealing with 30 cycles and resulted a right fragment of 379 bp. The fragments were digested with HindIII at 37°C during for four hours for genotyping analysis. The result showed that BB genotypes were found at the locus of CSN3 gene in all samples. Those BB genotypes merged the pattern of digested fragments with sizes of 255 and 154 bp. This finding suggests that genotyping using HindIII reveals monomorphics of BB types accociated with milk content of casein. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Center for Animal Science Research and Development (ICARD) en-US
dc.rights Copyright (c) 2017 Proceeding International Seminar on Livestock Production and Veterinary Technology en-US
dc.source Proceedings of International Seminar on Livestock Production and Veterinary Technology; 2016; 37-44 en-US
dc.title Genotyping of Kappa-Casein Gene of Buffalo in Indonesian en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US

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