Identifikasi cDNA Gen RB pada Tanaman Kentang Produk Rekayasa Genetika Katahdin SP951
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Date
2016-08-09
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Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
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To overcome the negative impact of the fungicide application, RB gene, originated from Solanum bulbocastanum, a resistancegene to the disease has been introduced to the genome of potato plant Katahdin, which was later named as Katahdin SP951.The aims of the research were to confirm the RB gene in the genetically modified plant (GMP) Katahdin SP951 throughresequencing the RB gene in the Katahdin SP951 genome and to align the RB genes isolated from the transgenic Katahdin SP951and the cloning vector pLCD0454, which was used the vector to transform Katahdin cultivar. The RB gene has high similarity toother resistance genes in potato plants, and therefore nontransgenic Katahdin, and Solanum bulbocastanum were used in theanalysis as the negative and positive control, respectively. Seven pairs of specific primers, which could differentiate the RB genewere used for gene sequencing studies. The sequencing experiments obtained 2913 bases which showed 100% similarity to theRB sequence isolated from the pLCD04541 plasmid. It is concluded that the RB gene in the Katahdin SP951 did not experiencemutation during transformation process and integration into the Katahdin genome.