Efforts to Develop Rapid Technology of In Vitro Transcription and Translation in Vaccine Synthesis in Indonesia

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dc.contributor en-US
dc.creator Ali, Muhamad
dc.date 2016-03-10
dc.date.accessioned 2018-06-04T07:49:33Z
dc.date.available 2018-06-04T07:49:33Z
dc.identifier http://medpub.litbang.pertanian.go.id/index.php/wartazoa/article/view/1228
dc.identifier 10.14334/wartazoa.v25i4.1228
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/4776
dc.description Production of functional protein (including vaccine) using conventional technology in embryonated chicken eggs is laborious and lengthy. The use of chemical synthesis is not practical for peptides longer than 20 residues. In contrast, in vitro transcription and translation technology can directly utilize polymerase chain reaction (PCR) product as template for vaccine synthesis within two hours accurately. Moreover, up to 1-10 mg/ml protein can be produced using the technology compared to conventional method that only gives approximately one dose per egg. In this review, advantages and disadvantages of animal vaccine generation using conventional and in vitro methods would be described. In vitro transcription and translation technology can be considered as the most practical and efficient technique for rapid screening and generating new animal vaccines. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Center for Animal Research and Development en-US
dc.relation http://medpub.litbang.pertanian.go.id/index.php/wartazoa/article/view/1228/1229
dc.source 2354-6832
dc.source 0216-6461
dc.source WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences; Vol 25, No 4 (2015): DECEMBER 2015; 181-188 en-US
dc.subject en-US
dc.title Efforts to Develop Rapid Technology of In Vitro Transcription and Translation in Vaccine Synthesis in Indonesia en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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