The Advance of Technology of Reverse Transcriptase-Polymerase Chain Reaction in Identifying the Genome of Avian Influenza and Newcastle Diseases

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dc.contributor en-US
dc.creator Hewajuli, Dyah Ayu; Balai Besar Penelitian Veteriner
dc.creator Dharmayanti, NLPI; Balai Besar Penelitian Veteriner
dc.date 2014-03-01
dc.date.accessioned 2018-06-04T07:49:18Z
dc.date.available 2018-06-04T07:49:18Z
dc.identifier http://medpub.litbang.pertanian.go.id/index.php/wartazoa/article/view/1022
dc.identifier 10.14334/wartazoa.v24i1.1022
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/4658
dc.description Avian Influenza (AI) viruses are zoonotic and caused death in humans. Newcastle Diseases (ND) virus has an economical impact in poultry. Therefore, the identification and characterization of AI and ND viruses that are appropriate, accurate and quick are important to protect human and poultry health. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) was the latest gold standard to detect the genome of AI and ND viruses. Recently, RT-PCR was developed in routine diagnosis and research. RT-PCR is a method to amplify the sequences of DNA genome, preceded by reverse transcriptase process with the primer-mediated enzymatic. Some factors that influenced detection of AI and ND are design primer and probe, types of samples, enzyme, reagent composition, amplification temperature and cycles, technical and non-technical factors such as contamination and trained staff. Modified conventional and real time RT-PCR are able to improve the specificity and sensitivity of the test. Key words: Detection, Avian Influenza, Newcastle Diseases, RT-PCR en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Center for Animal Research and Development en-US
dc.relation http://medpub.litbang.pertanian.go.id/index.php/wartazoa/article/view/1022/1027
dc.source 2354-6832
dc.source 0216-6461
dc.source WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences; Vol 24, No 1 (2014): MARCH 2014 en-US
dc.title The Advance of Technology of Reverse Transcriptase-Polymerase Chain Reaction in Identifying the Genome of Avian Influenza and Newcastle Diseases en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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