KINETIC EVALUATION OF ETHANOL-TOLERANT THERMOPHILE Geobacillus thermoglucosidasius M10EXG FOR ETHANOL PRODUCTION

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dc.contributor en-US
dc.creator Riyanti, Eny Ida; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development
dc.creator Rogers, Peter L.; bSchool of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, Australia
dc.date 2016-10-25
dc.date.accessioned 2018-05-02T01:18:06Z
dc.date.available 2018-05-02T01:18:06Z
dc.date.issued 2016-10-25
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/605
dc.identifier 10.21082/ijas.v10n1.2009.p24-41
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/43
dc.description Thermophiles are challenging to be studied for ethanol production using agricultural waste containing lignocellulosic materials rich in hexose and pentose. These bacteria have many advantages such as utilizing a wide range of substrates, including pentose (C5) and hexose (C6). In ethanol production, it is important to use ethanol tolerant strain capable in converting lignocellulosic hydrolysate. This study was aimed to investigate the growth profile of ethanol-tolerant thermophile Geobacillus thermoglucosidasius M10EXG using a defined growth medium consisted of single carbon glucose (TGTV), xylose (TXTV), and a mixture of glucose and xylose (TGXTV), together with the effect of yeast extract additionto the media. The experiments were conducted at the School of Biotechnology and Biomolecular Sciences of The University of New South Wales, Australia on a shake flask fermentation at 60°C in duplicate experiment. Cultures were sampled every two hours and analised for their kinetic parameters including the maximum specific growth rate (µmax), biomass yield (Yx/s), ethanol and by-product yields (acetate and L-lactate) (Yp/s), and the doubling time (Td). Results showed that this strain was capable of growing on minimal medium containing glucose or xylose as a single carbon source. This strain utilized glucose and xylose simultaneously (co-fermentation), although there was glucose repression of xylose at relatively low glucose concentration (0.5% w/v), particularly when yeast extract (0.2% w/v) was added to the medium. The highest biomass yield was obtained at 0.5 g l-1 on glucose medium; the yield increased when yeast extract was added (at 0.59 g l-1). The highest specific growth rate of 0.25 was obtained in the phase I growth when the strain was grown on a mixture of glucose and xylose (0.5% : 0.5% w/v) medium. Diauxic growth was shown on the mixture of glucose, xylose, and yeast extract. The strain produced low level of ethanol (0.1 g l-1), as well as low level (0.2 g l-1) of by-products (L-lactate and acetate) after 15 hours. The results suggests its potential application for fermenting lignocellulosic agricultural wastes for ethanol production. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Agency for Agricultural Research and Development en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/605/386
dc.source 2354-8509
dc.source 1411-982X
dc.source Indonesian Journal of Agricultural Science; Vol 10, No 1 (2009): April 2009; 24-41 en-US
dc.title KINETIC EVALUATION OF ETHANOL-TOLERANT THERMOPHILE Geobacillus thermoglucosidasius M10EXG FOR ETHANOL PRODUCTION en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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