Multiplikasi Tunas dan Konservasi In Vitro Tanaman Belitung (Xanthosoma sagittifolium (L.) Schott) dengan Metode Pertumbuhan Minimal

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dc.contributor en-US
dc.creator Sabda, Muhamad; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A Bogor 16111
dc.creator Dewi, Nurwita; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A Bogor 16111
dc.date 2018-02-13
dc.date.accessioned 2018-05-02T06:14:51Z
dc.date.available 2018-05-02T06:14:51Z
dc.date.issued 2018-02-13
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/8365
dc.identifier 10.21082/jbio.v12n2.2016.p101-108
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/436
dc.description In Vitro Shoot Multiplication and Conservation of Belitung (Xanthosoma sagittifolium (L.) Schott) using Minimal Growth Conservation Method. Muhammad Sabda and Nurwita Dewi. Belitung is one of potential food crops which has a lot of benefit. Belitung germplasm collection was conserved in the field which is vurnerable to biotic and abiotic stress.  The purpose of this research was to study the effect of  BAP on in-vitro shoot multiplication of belitung, and to study influences of osmoticum (mannitol) and retardan (paclobutrazol) to the growth of belitung culture. Experimental design used in this research was randomized completely design  with three  replications.  Shoot from sucker were used as explant. In study of shoot multiplication, the treatment medium was MS +  BAP (0, 1, 2, 3 mg/l). In study of conservation, the treatment medium was MS + 30 g/l sucrose, ½ MS + 30 g/l sucrose, MS + 15 g/l sucrose, MS + Mannitol (20,  40 and 60 g/l) and MS + Paclobutrazol (1, 2 and 3 mg/ l). The result showed that the addition of Benzyl Amino Purine (BAP) into MS medium increased number of shoot of belitung.  The best medium for micropropagation of belitung germplasm was MS + BAP 3 mg/l. The highest number of shoot (7.6 shoots) was produced by MS +  BAP 3 mg/l medium.  MS + mannitol (20 and 40 g/l) and MS + paclobutrazol (1, 2, and 3 mg/l) medium could reduce the  plant growth. MS + Paclobutrazol 2 mg/l was the best medium for belitung conservation. This media could reduce plant growth during eight months conservation period and prolong subculture interval. en-US
dc.format application/pdf
dc.language eng
dc.publisher Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/8365/7169
dc.rights Copyright (c) 2018 Jurnal AgroBiogen en-US
dc.rights http://creativecommons.org/licenses/by-nc-sa/4.0 en-US
dc.source 2549-1547
dc.source 1907-1094
dc.source Jurnal AgroBiogen; Vol 12, No 2 (2016): Desember; 101-108 en-US
dc.title Multiplikasi Tunas dan Konservasi In Vitro Tanaman Belitung (Xanthosoma sagittifolium (L.) Schott) dengan Metode Pertumbuhan Minimal en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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