Development of SSR Marker Set to Identify Fourty Two Indonesian Soybean Varieties

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dc.contributor en-US
dc.creator Risliawati, Andari; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Phone (+62-251) 8337975; Fax. (+62-251) 8338820
dc.creator Riyanti, Eny I.; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Phone (+62-251) 8337975; Fax. (+62-251) 8338820
dc.creator Lestari, Puji; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Phone (+62-251) 8337975; Fax. (+62-251) 8338820
dc.creator Utami, Dwinita W.; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Phone (+62-251) 8337975; Fax. (+62-251) 8338820
dc.creator Silitonga, Tiur S.; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Phone (+62-251) 8337975; Fax. (+62-251) 8338820
dc.date 2016-08-09
dc.date.accessioned 2018-05-02T06:14:48Z
dc.date.available 2018-05-02T06:14:48Z
dc.date.issued 2016-08-09
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3823
dc.identifier 10.21082/jbio.v11n2.2015.p49-58
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/409
dc.description Profile of molecular marker can be used for variety identification, genetic purity monitoring of germplasm and additionalrequirement in proposing intellectual property protection. DNA fingerprinting of soybean had been applied at the ICABIOGRADIAARDsince 2004 using simple sequence repeat (SSR) markers which were run automatically by CEQ 8000 Genetic Analyzerplatform based on capillary electrophoresis system. This method had produced unique DNA fingerprints of the varieties tested,but the marker set to efficiently identify the varieties had not yet been developed. This study aimed to develop a set of SSRmarkers as a tool to identify the Indonesian soybean varieties. Fourty two soybean varieties were analyzed using 14 random SSRmarkersA total of 168 alleles that were obtained from the polymorphism analysis. The average of polymorphic informationcontent (PIC) value observed was 0.7337 per SSR locus. Based on marker reproducibility rate, PIC value, number of rare alleles,frequency of dominant alleles, and percentage of SSR fragment detected by genetic analyzer, we identified five SSR markers i.e.Satt414, Satt147, Satt308, Satt009, and Satt516 as a SSR marker set to be used for soybean variety identification purposes. Thismarker set was used to develop the identity (ID) of the 42 Indonesian soybean varieties. en-US
dc.format application/pdf
dc.language eng
dc.publisher Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3823/3172
dc.rights Copyright (c) 2016 Jurnal AgroBiogen en-US
dc.source 2549-1547
dc.source 1907-1094
dc.source Jurnal AgroBiogen; Vol 11, No 2 (2015): Agustus; 49-58 en-US
dc.title Development of SSR Marker Set to Identify Fourty Two Indonesian Soybean Varieties en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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