The study aims to determine the effectiveness of acetic acid based formula on broad leaf weeds grown in medicinal crop fields. Two experiments were conducted, i.e. on small plots (2 m x 3 m) of a farmer’s field in Ciapus, Bogor and on a larger plot (2 m x 8 m) at the Cicurug Experimental Station, Sukabumi. The formula consisted of a mixture of acetic acid + NaCl (AG) and acetic acid + citric acid (AC), and formulas of VAC, VACG which were enriched with wood vinegar (V). As control was a commercial synthetic herbicide 2,4 D amine. The acetic acid base formulas applied at two concentrations 10 and 15%, while 2,4-diamine was followed the recommendation (0.3%) using a knapsack sprayer with a flat-fan nozzle tip. Weed vegetations were observed visually before and at 2, 4, 6, and 8 weeks after treatments. Weed severity was recorded using a 0-4 scales (0 = 0-5% mortality ; 1 = 20-50% weeds died ; 3 = 50-75% eradicated and 4 = 75-100% weed were eradicated), as well as re-growth and dry weight of the weeds. The first and second experiment showed that AC, AG, VAC and VACG applied at 10 and 15% were effective in controlling broadleaf weeds. Mechanism of action the formula is a contact poison. Its effectiveness is equivalent to 2,4-D amine treatment dose of 1.5 l ha-1. Repeated application is necessary to prolong the effect of herbicide.