Effectivity of PCR and AGID methods to detect of enzootic bovine leukosis in Indonesia

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dc.contributor en-US
dc.creator M, Saepulloh
dc.creator I, Sendow
dc.date 2015-03-12
dc.date.accessioned 2018-06-04T06:48:35Z
dc.date.available 2018-06-04T06:48:35Z
dc.date.issued 2015-03-12
dc.identifier http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1120
dc.identifier 10.14334/jitv.v20i1.1120
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/3385
dc.description Enzootic Bovine Leucosis (EBL) is one of viral diseases in cattle caused by bovine leukemia virus (BLV), from Retroviridae. The virus can be detected using severals methods such as Polymerase Chain Reaction (PCR), while antibody can be detected using Agar Gel Immunodifussion (AGID). The aim of this experiment was to study the effectivity of PCR and AGID methods to detect enzootic bovine leukosis virus in Indonesia. Samples of peripheral blood leukocyte (PBL) were collected from cattles those with and without showing clinical signs. A total of 307 blood  and serum samples were  tested against BLV using PCR and AGID tests, while 21 semen samples which were from similar animals for blood collection were collected only for PCR test. The results indicated that twelve cattles have positive  results with PCR test in PBL, but from those cattles  only seven were positive with AGID. On the other hand, the PCR did not detect EBL in 21 bovine semen samples tested, although one sample gave positive result with PCR in PBL. This results indicated that PCR method from blood samples was more sensitive than that AGID method. The PCR detection was also more sensitive for PBL than that for semen samples. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Animal Sciences Society en-US
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1120/pdf
dc.rights Copyright (c) 2015 Indonesian Journal of Animal and Veterinary Sciences en-US
dc.rights http://creativecommons.org/licenses/by/4.0 en-US
dc.source 2252-696X
dc.source 0853-7380
dc.source Indonesian Journal of Animal and Veterinary Sciences; Vol 20, No 1 (2015): MARCH 2015; p.71-78 en-US
dc.title Effectivity of PCR and AGID methods to detect of enzootic bovine leukosis in Indonesia en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US

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