Effects of glycerol in tris extender on frozen semen quality of crossbred Etawah bucks

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dc.creator Tambing, Surya Natal
dc.creator Toelihere, Mozes R
dc.creator Yusuf, Tuty L
dc.creator Sutama, I ketut
dc.date 2014-02-17
dc.date.accessioned 2018-06-04T06:47:54Z
dc.date.available 2018-06-04T06:47:54Z
dc.date.issued 2014-02-17
dc.identifier http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/203
dc.identifier 10.14334/jitv.v5i2.203
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/3080
dc.description Bucks semen is easily damaged during freezing process, due to the formation of ice crystals. Consequently, semen quality decreases particularly the post-thaw sperm motility, viability, intack plasma membrane and intack acrosomal cap. The objectives of this research are to determine the optimum dose of glycerol in Tris extender in maintaining frozen semen quality of crossbred Etawah bucks. Four head of PE buck of 2 - 4 years old were used in this experiment. Doses of glycerol used were 5%, 6% and 7%. Semen was collected once a week using artificial vagina. Results of this experiment indicated that the mean percentage of pre-freezing motility, live sperm, sperm with intact plasma membrane and intact acrosomal cap in 5%, 6% and 7% glycerol were not significantly different (P>0.05). After freezing, the mean percentage of motility, live sperm, sperm with intact plasma membrane and intact acrosomal cap in 6% glycerol (52.60%, 65.03%, 45.63% and 47.54% respectively) were significantly higher (P<0.05) than in 5% (44.31%, 52.00%, 37.60% and 37.00% respectively) and in 7% glycerol (45.28%, 52.10%, 37.97% and 37.14% respectively). However, there were not significant differences in 5% and 7% glycerol for any parameter measured. It was concluded that supplementation of glycerol 6% in Tris extender effective to protect of sperm from various shock during the process of semen cryopreservation, so that it could maintain of frozen semen quality (sperm motility, viability, intack plasma membrane and intack acrosomal cap) that suitable used in AI program of crossbred Etawah bucks.   Key words : Glycerol, PE bucks, semen quality en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Animal Sciences Society en-US
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/203/203
dc.rights Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences en-US
dc.rights http://creativecommons.org/licenses/by/4.0 en-US
dc.source 2252-696X
dc.source 0853-7380
dc.source Indonesian Journal of Animal and Veterinary Sciences; Vol 5, No 2 (2000): JUNE 2000; p.84-91 en-US
dc.title Effects of glycerol in tris extender on frozen semen quality of crossbred Etawah bucks en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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