Morphological diversity and genetic differentiation of PO cattle in smallholder farmers

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dc.creator ., Hartati
dc.creator ., Sumadi
dc.creator ., Subandriyo
dc.creator Hartatik, Tety
dc.date 2012-03-02
dc.date.accessioned 2018-06-04T06:47:48Z
dc.date.available 2018-06-04T06:47:48Z
dc.date.issued 2012-03-02
dc.identifier http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/680
dc.identifier 10.14334/jitv.v15i1.680
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/3045
dc.description PO cattle is one of  the local cattle with high genetic diversity. The aim of this research was to study genetic diversity of PO cattle in smallholder farmer  based on morfology and molecular markers. This research was conducted at breeding center in East Java and Central Java include Tuban, Lamongan and Blora regency, since June until December 2008.  PO bull used were of 18 months until 24 months of age and cow of 24 months until 36 months of age or at first calving as much as 30 head each location. The variables measured were body weight, body length, withers height, hip height, shoulder point width, chest girth, chest depth, canon bone circumference, head length and head width. Data were analyzed using descriptive statistic, average analysis between subpopulations, discriminant and canonical analysis and mahalanobis distance was done by SAS DISCRIM procedure. Whole blood were collected to get the data of polymorphism DNA microsatellite using technology of PCR and electrophoresis using 6 microsatellite loci. Variable measured were number and size of  alel, alel frequency, heterozygosity,genetic distance and fixation index (F-stat). Data were analyzed using analysis of Microsate Toolkit, GENEPOPV4, MSA (Microsatellite Analyser) and Mega 4. The result showed that Blora and Tuban subpopulation have genetic diversity which is relative lower  compared to that of Lamongan. The genetic distance which was close presented by Tuban and Blora subpopulation while Lamongan and Tuban subpopulation show far genetic distance. The result of canonical analyses showed  high correlation on shoulder point width, head width, body length, withers height and hip height variable so this five variable can be used as distinguishing variables among subpopulation. The result of molecular genetics analysis using microsatellite showed that highest frequency of alel presented by HEL9 locus at Tuban population so this loci have the high polymorfism. The genetic differentiation among subpopulation showed by differentiation FST value among six loci  indicating the increasing of inbreeding in  the three subpopulations. Key words: Morphological Diversity, Genetic Differentiation, PO Cattle en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Animal Sciences Society en-US
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/680/689
dc.rights Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences en-US
dc.rights http://creativecommons.org/licenses/by/4.0 en-US
dc.source 2252-696X
dc.source 0853-7380
dc.source Indonesian Journal of Animal and Veterinary Sciences; Vol 15, No 1 (2010): MARCH 2010; p.72-80 en-US
dc.title Morphological diversity and genetic differentiation of PO cattle in smallholder farmers en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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