Stallion semen cryopreservation using different cryoprotective agents on the skim milk trehalosa extender

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dc.creator Arifiantini, R.I
dc.creator Supriatna, I 2012-02-05 2018-06-04T06:47:47Z 2018-06-04T06:47:47Z 2012-02-05
dc.identifier 10.14334/jitv.v12i2.552
dc.description Cryoprotective agents (CPAs), protect the sperm during cryopreservation. There are two general classes of CPAs, at first penetrating cryoprotectants, these pass through the sperm membrane and act both intracellular and extracellularly, and the second non-penetrating cryoprotectants, these act only extracellularly. The objective of this study was to evaluate the addition of different CPAs namely glycerol (G), combination of ethylene glycol with glycerol (EG) and dimethilformamide (DMF) using skim milk trehalosa extender. The semen collected from 3 stallions using artificial vagina twice aweeks. The semen was evaluated, centrifugated and diluted in skim milk extender supplemented with 50 mM trehalose and three different CPAs with the concentration of sperm were 200x106 ml-1. Extended semen was then packed into minitub 0.3 ml and equilibrated at 4oC for 2 hours, freeze in the liquid nitrogen vapor for 10 minutes and stored in liquid nitrogen container -196oC. After 24 hours, the semen was thawed at 37oC for 30 second. Results of this experiment indicated that the percentage of motile and viable sperm in skim trehalosa extender using DMF and glycerol better than combination of ethylene glycol and glycerol.   Key Words: Cryopreservation, Stallion Sperm, Cryoprotectant en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Animal Sciences Society en-US
dc.rights Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences en-US
dc.rights en-US
dc.source 2252-696X
dc.source 0853-7380
dc.source Indonesian Journal of Animal and Veterinary Sciences; Vol 12, No 2 (2007): JUNE 2007; p.139-146 en-US
dc.title Stallion semen cryopreservation using different cryoprotective agents on the skim milk trehalosa extender en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US

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