The comparative study of antigenic protein characters of field isolates Brucella abortus cells with electrophoresis and immunoblotting Techniques

Show simple item record

dc.contributor en-US
dc.creator Sudibyo, Agus
dc.creator Pasaribu, Facriyan H
dc.creator Wibawan, I.W.T.
dc.creator Setiawan, Endhie
dc.date 2014-02-25
dc.date.accessioned 2018-06-04T06:47:18Z
dc.date.available 2018-06-04T06:47:18Z
dc.date.issued 2014-02-25
dc.identifier http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/32
dc.identifier 10.14334/jitv.v1i3.32
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/2830
dc.description Live vaccine of Brucella abortus strain 19 has been used for prevention and eradication of brucellosis in cattle . The information of the use of this vaccine in Indonesia is still limited, while the effectivity, bacteriological and serological aspects of the vaccine are not much evaluated yet. The objective of this research is to study the differences of protein cell wall antigenicity profiles between B. abortus strain 19, strain 544 and field isolates. Protein cell wall was prepared by sonicafion of B. abortus S19, S544 and B. abortus field isolates biotype l, 2 and 3 . Antiserum against these B. abortus was prepared in cattle . Futhermore, the distribution of protein band was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), while protein antigenic profiles was examined by immunoblotting . The result showed that there was no significant different amongs protein cell wall of these B. abortus. From the antigenicity profiles exhibited that cattle vaccinated with B. abortus S19, no antibody was detected against protein less than 30 kDa. The other side natural infected or experimental infected cattle with B. abortus biotype 1 field isolate, antibody was detected until protein which has molecular weight about 15 kDa. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Animal Sciences Society en-US
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/32/32
dc.rights Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences en-US
dc.rights http://creativecommons.org/licenses/by/4.0 en-US
dc.source 2252-696X
dc.source 0853-7380
dc.source Indonesian Journal of Animal and Veterinary Sciences; Vol 1, No 3 (1995); p.185-189 en-US
dc.title The comparative study of antigenic protein characters of field isolates Brucella abortus cells with electrophoresis and immunoblotting Techniques en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search Repositori


Browse

My Account