Effect of Bali cattle ovarian status on oocytes nuclear maturation and in vitro fertilization rate

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dc.contributor en-US
dc.creator Sonjaya, Herry; Hasanuddin University
dc.creator Yusuf, M.; Department of Livestock Production, Faculty of Animal Science, University of Hasanuddin, Jl. Perintis Kemerdekaan Km.10, Makassar 90245, Indonesia
dc.creator Hamdana, A.; Department of Livestock Production, Faculty of Animal Science, University of Hasanuddin, Jl. Perintis Kemerdekaan Km.10, Makassar 90245, Indonesia
dc.creator Utamy, Renny Fatmyah; Hasanuddin University
dc.creator Gustina, Sri; Sulawesi Barat University
dc.creator Hasbi, Hasbi; Hasanuddin University
dc.date 2018-03-05
dc.date.accessioned 2018-06-04T06:47:00Z
dc.date.available 2018-06-04T06:47:00Z
dc.date.issued 2018-03-05
dc.identifier http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1585
dc.identifier 10.14334/jitv.v22i4.1585
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/2692
dc.description The aim of this study was to investigate whether the reproductive status influences the nuclear maturation and fertilization rates of bali cattle oocytes in vitro. Several pairs of ovary were classified into four groups: 1) ovaries with Corpus Luteum (CL) and Dominant Follicle (DF), 2) ovaries without CL and with DF, 3) ovaries with CL and without DF, 4) ovaries without both CL and DF. In the first experiment, oocytes were collected by slicing method in Phosphate Buffer Saline (PBS) medium supplemented with 10% Fetal Bovine Serum (FBS) and 100 IU/ml penicillin streptomycin. Oocytes were matured in tissue culture medium (TCM)-199 supplemented with 10% Fetal Bovine Serum (FBS), 10 IU/ml Follicle Stimulating Hormone (FSH), 10 IU/ml Luteinizing Hormone (LH), and 50 μg/ml gentamycin. Oocytes were matured in 5% CO2 incubator, 38oC for 24 h. In the second experiment, oocytes were matured and then fertilized in vitro to observe pronuclear formation. The first experiment showed that the percentage of oocytes reached methaphase-II (MII) stage on ovaries with CL and without DF (89.47%) were higher (P<0,01) compared to ovaries without both CL and DF (75,47%), ovaries without CL and with DF (74.,41%), or ovaries with CL and DF (65,52%). The result of second experiment showed that the ovarian reproductive status was not significantly different (P>0.05) on fertilization rate. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Animal Sciences Society en-US
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1585/1570
dc.rights Copyright (c) 2018 Indonesian Journal of Animal and Veterinary Sciences en-US
dc.source 2252-696X
dc.source 0853-7380
dc.source Indonesian Journal of Animal and Veterinary Sciences; Vol 22, No 4 (2017): DECEMBER 2017; 173-178 en-US
dc.title Effect of Bali cattle ovarian status on oocytes nuclear maturation and in vitro fertilization rate en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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