Response of Five Kenaf Accessions to Shoot Regeneration
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Date
1999
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Publisher
Pusat Penelitian dan Pengembangan Perkebunan
Abstract
This experiment was aimed to study response of fives kenfa accession
to shoot regeneratioo and to establish an appropriate regeaeraiea protocol for
kenaf The experiment was performed at Tissue Culture Laboratory Research
Institute for Tobacco and Fibre Crops (RITFC), MaJang, from June to Octo-
ber 1997. Crtyledons with plumules attached were used as explants and
culture medium for callus induction was MS-based medium with BAP (2
mgfl) and NAA (0.5 mgfl). Calli produced in this cultures were transferred
into MS-based medium containing BAP (2 mgfl) and GAJ (5 mgfl) for shoot
initiatioo. All shorts obtained were then sub-cultured in MS-based medium
without regulators (MSO) for root formation. Rooted shorts (plantlets) were
acclimatized in the sterile sand and transferred into sterile soil in the glass
house. Results of this experiment showed that the most respoosive accessions
were Cuba 10811, followed by KK 60, He 48, PI 324922, aid CRNI 056 H
with the average number of shoots per explant 4.32 ± 4.21, 4.00± 4.01, 3.05
± 2.98, 2.80± 1.47, and 2.72 ± 2.49, respectively, at 40 days after transferring
on shoot regeneratica medium. Shoots rooted after 14 days on MSO medium
with frequencies of 81.50-93.30%. Healthy plaatlets survived and grew well
in soil media in the glass house. Therefore, an appropriate short regeneration
protocol for kenafwas found,
Description
Keywords
Hibiscus cannabinus L.,, shoots regeneration,, tissue culture