Improving the Effects of Omega-3 Fatty Acid on the In Vitro Maturation of Oocytes

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dc.contributor en-US
dc.creator Ghorbani Vahed, Masume; Department of Biology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
dc.creator Khanbabaee, Ramazan; Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
dc.creator Shariati, Mehrdad; Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
dc.creator Edalatmanesh, Mohammad Amin; Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran 2020-12-14 2021-09-07T02:48:04Z 2021-09-07T02:48:04Z
dc.identifier 10.14334/jitv.v25i4.2555
dc.description This research was conducted in order to determine the effects of omega-3 on oocyte in vitro maturation and the level of expression of tribbles (TRIB1, TRIB2 and TRIB3 genes) in cumulus cells. Eight-ten weeks old NMRI mice were super-ovulated using 7.5 IU pregnant mare’s serum gonadotropin (PMSG, Intraperitoneal) and they were killed after 44 hours and their ovaries were removed. The oocytes were used for in vitro maturation and the cumulus-oocyte complexes (COCs) were released. Cumulus cells and oocytes were assigned into control, ethanol-treated and groups exposed to 10 and 100 μg/ml of omega-3. The cells were prepared to assess the maturation stage in order to evaluate the gene expression level. The data were statistically analyzed. Exposing oocytes to low dose (10 μg/ml) and high dose (100 μg/ml) of omega-3 resulted in a reduced rate of GV-stage oocytes, decreased MI-oocytes and increased MII-oocytes. The enhanced maturity of COCs was also detected in response to a high dose of omega-3 (100 μg/ml). Exposure of cumulus cells to omega-3 (10 and 100 μg/ml) induced TRIB2 and inhibited TRIB3 gene expression level; however, TRIB1 gene expression level increased and decreased in response to low (10 μg/ml) and high (100 μg/ml) concentrations of omega-3, respectively. The addition of omega-3 to the environment of oocytes or cumulus cells affected the maturation of oocytes and cumulus cells, which was followed by the differential expression of TRIB genes, suggesting that there was a role of fatty acid metabolism in the differentiation and maturation of cumulus cells. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Center for Animal Research and Development (ICARD) en-US
dc.rights Copyright (c) 2020 Jurnal Ilmu Ternak dan Veteriner en-US
dc.source Jurnal Ilmu Ternak dan Veteriner; Vol 25, No 4 (2020): December 2020; 153-161 en-US
dc.source 2252-696X
dc.source 0853-7380
dc.subject Cumulus Cells, Maturation, Omega-3, Oocyte, TRIB Genes en-US
dc.title Improving the Effects of Omega-3 Fatty Acid on the In Vitro Maturation of Oocytes en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US

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