Point mutation of ITS-nrDNA sequences as specific markers of three durian species: Durio zibethinus, D. kutejensis and D. lowianus
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Date
2020
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IAARD Press
Abstract
Molecular markers are considered more efficient tools than morphological markers for species identification. This research aimed to get specific molecular markers among three durian species Durio zibhetinus, D. kutejensis and D. lowianus using ITS-nrDNA sequences. A number of 139, 7 and 1 ITS sequences of D. zibethinus, D. kutejensis and D. lowianus, respectively, were used in this experiment. Each group of sequences was then aligned to make one consensus sequence. In order to identify the point mutations, the aligned sequences were cut with restriction enzyme in silico using Genious ver. 7 software. The simulations found that
each consensus sequences has different point mutation forming different restriction site. Consensus sequences of D. zibethinus has EcoR1 site on base number 280, D. kutejensis has Ama871 site on base number 394 and D. lowianus has Aco1 site on base number 135. These signals could be used as specific markers for the three durian species
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Keywords
Durio, ITS-nrDNA, point mutation, species markers.