Information System and Exchanges of Genetic Resources for Effective Crop Improvementhttps://repository.pertanian.go.id/handle/123456789/12016https://repository.pertanian.go.id/retrieve/5362a05c-a618-4661-9f97-f010e68bc2c6/2024-03-19T10:17:09Z2024-03-19T10:17:09Z501Characterization of 337 exon-based single nucleotide polymorphisms (SNPs) unique to the Indonesian soybean varietieshttps://repository.pertanian.go.id/handle/123456789/120862021-03-17T07:14:43Z2020-01-01T00:00:00Zdc.title: Characterization of 337 exon-based single nucleotide polymorphisms (SNPs) unique to the Indonesian soybean varieties
dc.description.abstract: Genome resequencing of five Indonesian soybean varieties resulted in a total of 2,597,286 single nucleotide polymorphisms (SNPs), 257,598 insertions, and 202,157 deletions. Out of those SNPs, only 95,207 (2.15%) were located in the protein-coding region (exon). The objective of this study was to characterize 337 exon-based SNPs unique to the Indonesian soybean varieties. The study was conducted by taking SNP samples located in the exons using criteria of gene fragments containing the SNPs that were sequenced at least five times within each of the soybean varieties. Out of 95,154 gene-based SNPs detected, only 337 SNPs met the criteria. Each of the soybean varieties was genotyped with the 337 SNP loci, and the genotypic data were scored and analyzed. Results showed that 59 SNPs were common to all five soybean genotypes. A total of 43, 41, 25, 32 and 28 SNPs loci were unique to soybean genotype Davros, Grobogan, Malabar, Tambora and B3293, respectively. These unique SNPs can function as DNA fingerprints for each variety. Out of 59 common SNPs, 24 SNPs were mutations that change the amino acid sequence of the encoded proteins. These genes with amino acid change may have high economic values such as those controlling soybean adaptation in tropical climate, photoperiod insensitivity, disease and insect resistance genes. Expression analyses of the genes with amino acid change showed variation in the expression pattern across different soybean tissues. Functional genomic analysis is necessary to isolate genes useful for breeding purposes.
2020-01-01T00:00:00ZInventory and morphological characterization of local upland rice in the highlands of South Sumatra provinceSasmita P. ...[at al]https://repository.pertanian.go.id/handle/123456789/120852021-03-17T07:11:03Z2020-01-01T00:00:00Zdc.title: Inventory and morphological characterization of local upland rice in the highlands of South Sumatra province
dc.contributor.author: Sasmita P. ...[at al]
dc.description.abstract: South Sumatra has various types of local rice in various agroecosystems that have the potential as plant genetic resources (PGR) of food crops. At present, the existence of the PGR has been diminished due to genetic erosion. Inventory and characterization of local rice genetic resources were carried out in the highlands of South Sumatra during 20142017. The study was conducted in Muara Enim, Ogan Komering Ulu, Pagar Alam, and Lahat Regencies. The objective of the study was to inventory and characterize the PGR. The method used was exploration and desk study. Purposive sampling was applied. Morpho-agronomic characterization was carried out in situ and ex situ. Morphological observations were carried out by visually observing the parts of leaves, stems, panicles and unhulled seeds of the rice accessionsin the location. Morphological characteristics were analyzed descriptively by tabulation. Inventory result showed 17 varieties of local upland rice were spread across four regencies. Local upland rice varieties originating from the regencies with different biophysical characteristics showed differences in morphological characteristics. Among the 17 local varieties, 3 varieties were characterized, namely Setangkai, Barokah and Ayek Keruh. Morpho-agronomic characterization results showed that there was a high diversity of rice accessions collected from different locations in South Sumatra.
2020-01-01T00:00:00ZThe performance of growth and yield component of soybean varieties in Margodadi village, Ambarawa sub-district, Pringsewu regency, Lampung province, IndonesiaMustikawati D R. ...[at al]https://repository.pertanian.go.id/handle/123456789/120842021-03-17T07:05:38Z2020-01-01T00:00:00Zdc.title: The performance of growth and yield component of soybean varieties in Margodadi village, Ambarawa sub-district, Pringsewu regency, Lampung province, Indonesia
dc.contributor.author: Mustikawati D R. ...[at al]
dc.description.abstract: Efforts to increase soybean production require superior varieties which are stable in every agroecology production center. Therefore, it is necessary to perform field evaluation of several new released soybean varieties. The aim of this study was to evaluate the growth and yield potential of soybean varieties developed by Indonesian Legume and Tuber Crops Research Institute (ILETRI) Malang in a field experiment. Four soybean varieties (Devon 1, Dering 1, Gema and Gepak Kuning) were sown in Margodadi Village, Ambarawa Sub-district, Pringsewu Regency, Lampung Province, Indonesia from May to August 2017. Plots were arranged in randomized complete block design with six replicates. The variables observed were crop emergence at 7 DAS sowing, plant height and number of pods at harvest, pest attack on 1- month-old plants and at harvest, and seed yield. The results showed that Gepak Kuning gave the highest yield (2.13 t/ha), which indicated that this variety is suitable to be planted and developed in the location of the study.
2020-01-01T00:00:00ZScreening genetic resources of local accessions of Capsicum originated from East JavaPurnomo S. ...[at al]https://repository.pertanian.go.id/handle/123456789/120832021-03-17T07:02:29Z2020-01-01T00:00:00Zdc.title: Screening genetic resources of local accessions of Capsicum originated from East Java
dc.contributor.author: Purnomo S. ...[at al]
dc.description.abstract: Inventory and collection of local chili varieties in production centers in East Java had been carried out in the period 2013–2015 by East Java Assessment Institute for Agricultural Technology (AIAT). A total of 143 accessions of five chili species (Capsicum annuum L., C. frutescens L., C. chinense Jacq., C. baccatum L. and C. pubescens L) was collected from eight chili production centers in East Java and has been maintained by East Java AIAT. A set of 85 local chili accessions was selected and evaluated for agronomic performance and biochemical compounds. Among the five species, C. annuum L. and C. frutescens L. dominated the distribution of chili species in East Java. The other three species were still found in some spots of farm households and highlands. C. pubescens, locally known as “Bodong” or “Wudel” chili, was found on the high slopes of Mount Semeru, whereas C. chinense, known as “Cotoh” chili by the locals, was also found in the highlands of Batu City. Based on agronomic performance there were several accessions with high-yielding potential (≥12 t/ha). Five accessions of C. frutescens collected from planting areas in the dryland with dry climate of Blitar not only had high yield potential, but also had high capsaicinoid content, low-fat content, low quercetin and high flavonoids and polyphenols. Phenotypic diversity and geographic origin may be useful as the criteria for selecting a good set of chili accessions.
2020-01-01T00:00:00ZDevelopment of physical color reference for Indonesia paddy collectionHidayatun N. ...[at al]https://repository.pertanian.go.id/handle/123456789/120822021-03-17T06:57:40Z2020-01-01T00:00:00Zdc.title: Development of physical color reference for Indonesia paddy collection
dc.contributor.author: Hidayatun N. ...[at al]
dc.description.abstract: One of the objectives of genebank management is to maintain the genetic identity of its collection. In order to maintain the genetic identity, it is important to check new seed against a reference collection. A simple morphological character for determining the reference collection is the color of lemma and palea. Lemma and palea is a pair of bract-like organ in the floret and have similar pattern of pigmentation and therefore can be treated together. The color of lemma and palea determine the seed color. To develop reference collection for grain color characterization of rice germplasm in the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD) Genebank, 1,000 sample of seeds produced from different year of regeneration are characterized based on the lemma and color categories of the IRRI guidelines for the characterization and evaluation system. Samples of each category were then arranged and photographed. From 11 categories of lemma and palea color described in the guidelines, there are six color categories available from genebank rice collection, whereas five color categories were not found. The six available color categories were straw, golden, brown spot in the straw background, brown line in the straw background, brown, orange-brown and black. Five color categories were not found in the collections i.e. red-purple, purple spots on straw, purple line on straw, purple and white. This material reference could be used for characterization of Indonesian rice. The seeds of the absent categories might need to be aquisitated to the genebank to complete the reference collection.
2020-01-01T00:00:00ZAnimal genetic resources (AnGR) in MozambiqueCumbula D. ...[at al]https://repository.pertanian.go.id/handle/123456789/120812021-03-17T06:53:42Z2020-01-01T00:00:00Zdc.title: Animal genetic resources (AnGR) in Mozambique
dc.contributor.author: Cumbula D. ...[at al]
dc.description.abstract: Mozambique is a country on the eastern coast of Southern Africa, where livestock plays an important role in the agriculture sector, due to its contribution to socio-economic development and poverty reduction. Given the variations in climate, soil fertility, rainfall pattern and altitude among the different areas, the country is divided into ten agroecological regions, each one with its own characteristic production systems and livestock breeds. In the family sector, livestock is mostly composed of indigenous breeds: Landim cattle, goats, pigs and chicken, Angoni and Bovino de Tete cattle breeds and Pafuri goat. In order to restore the quantity and quality of the genetic resources of indigenous breeds and promote their conservation and sustainable use, the country is carrying out several actions and activities, including public awareness campaigns about the importance of indigenous breeds and the need for their conservation, inclusion of AnGR issues in the curriculum of universities and agricultural colleges, characterization of AnGR collection and conservation of semen from native bulls.
2020-01-01T00:00:00ZDiversity of pod weight of Indonesian local groundnut (Arachis hypogaea L.) varieties at different harvesting timeZulchi T. ...[at a]https://repository.pertanian.go.id/handle/123456789/120802021-03-17T06:46:30Z2020-01-01T00:00:00Zdc.title: Diversity of pod weight of Indonesian local groundnut (Arachis hypogaea L.) varieties at different harvesting time
dc.contributor.author: Zulchi T. ...[at a]
dc.description.abstract: Groundnut, a self-pollinated legume, is an important cash crop which has high oil seed content and provides nutritious fodder to livestock. Harvesting time is very critical to the groundnut production, because their maturity determines the pod weight. This study aimed to observe the pod weight of local groundnut varieties of Indonesia at different harvesting time. A total of 50 local varieties groundnut were grown at ICABIOGRAD Experimental Station from May–September 2015. The pods were harvested at 75, 85 and 95 days after planting (DAP) and weighed. The results showed that the accessions reached its physiological maturity at 85 DAP, where the plants reached the highest maturity index (2.28). The average maturity index at 75 and 95 DAP was 1.64 and 1.84, respectively. The pod weightat 75, 85 and 95 DAP ranged from 1.0 to 31.4 g, 4.2 to 35.2 g and 2.0 to 31.6 g, respectively, whereas the seed weight ranged from 1.0 to 20.8 g, 2.2 to 26.4 g and 1.4 to 24 g, respectively. A relatively high seed and hull weight ratio at 85 DAP was found in MLG 1629 Jombang, MLG 7552 Gresik, Hoi Ambon, Lanbau and AH 842Si varieties. The harvesting time should be done at physiological maturity to identify the maturity type among local varieties. It is recommended that the Indonesian local varieties of groundnuts should be harvested at 85 DAP.
2020-01-01T00:00:00ZThe problems of ex situ genetic conservation at the universities in developing countries: lesson learn from Universitas Gadjah MadaTaryono ...[at al]https://repository.pertanian.go.id/handle/123456789/120792021-03-17T06:43:31Z2020-01-01T00:00:00Zdc.title: The problems of ex situ genetic conservation at the universities in developing countries: lesson learn from Universitas Gadjah Mada
dc.contributor.author: Taryono ...[at al]
dc.description.abstract: Agriculture faces enormous challenges for providing sufficient food, feed and fuel raw materials for a growing global population. In the case of food, for instance, global food production must always increase to meet the projection of continuously increase of global food demand. The future challenges of food supply and demand can be addressed by diversification of food sources, introducing high yielding cultivars and improving culture techniques. Food sources can be diversified by collection and evaluation of genetic resources for nutritive values. In contrast, new varieties can be developed through breeding activities that also require genetic resource as genetic material. Genetic resources spread around the world, and to optimally utilize, genetic resource must be explored and conserved both by in situ and ex situ approaches. The genetic resource exploration through missions requires proper preparation including human resources, logistics and time allocations. Universitas Gadjah Mada (UGM) as a higher education institution has three big university missions, i.e. education, research and community service through student involvement. These three missions through student involvement have
been applied to conduct the genetics resource exploration and ex situ conservation. The course of genetic resource collection and management has been introduced at different faculties, and because community service at the rural area for two-month times is compulsory for the student, UGM makes use of student to carry out genetic resource exploration and collection. The student must collect the passport data for the genetic resources and send the data to the Agriculture Innovation Center (AIC). In case that seed of genetic material can be found,
student must collect also seeds and send to AIC for ex situ conservation. Based on UGM experience, ex situ conservation, especially seed genebank, faced sustainability problem due to insufficient human and funding resources. UGM integrates some approaches such as crop focusing, networking, student involvement in the characterization and evaluation, and breeding activities to solve such problems.
2020-01-01T00:00:00ZSeed characteristics of local rice accessions from East Barito regencySusilawati ...[at al]https://repository.pertanian.go.id/handle/123456789/120782021-03-17T06:40:28Z2020-01-01T00:00:00Zdc.title: Seed characteristics of local rice accessions from East Barito regency
dc.contributor.author: Susilawati ...[at al]
dc.description.abstract: Central Kalimantan has an abundance of local rice genetic resources scattered in every district. One of the districts with important local rice genetic resources is East Barito. This study aimed to seek unique characters ol local rice varieties in East Barito Regency which could be useful for further genetic improvement. Local rice exploration was conducted during January to February 2018 to obtain local species in the East Barito District. The method of activity is purposive sampling by selecting 45 villages within the district based on information of diverse local genetic resources. The data collected consisted of the passport data and the present status of local paddy rice, as well as the character of the seeds to ensure that there was a difference of accession for local paddy genetic resources with the same name. Based on the exploration activity, a total of 27 local rice accessions have been explored from the region, and there were two cultivar local rice accessions that have the same name, i.e. Cantik Manis and Siam Cantik. However, they have different grain shape and other morphological characters.
2020-01-01T00:00:00ZTiller profile diversity of upland rice germplasm in ICABIOGRAD gene bankhttps://repository.pertanian.go.id/handle/123456789/120772021-03-17T06:36:50Z2020-01-01T00:00:00Zdc.title: Tiller profile diversity of upland rice germplasm in ICABIOGRAD gene bank
dc.description.abstract: Tillering ability is an important agronomic trait that determines the yield of rice. Tiller type in rice is categorized as primary, secondary and tertiary tiller which produces panicle at generative phase. Tillering ability as well as the tiller type varies between varieties, especially among germplasms. Our study aimed to identify the tiller profile diversity of upland rice germplasm in ICABIOGRAD Genebank. A total of 100 accessions of local upland rice varieties were planted in a randomized completely block design with two replications under greenhouse condition. The number of tiller from each type was observed weekly from 35 to 63 days after sowing (DAS). The result of the study showed that the number of primary tiller increased slowly from 35 DAS until 63 DAS. After 49 DAS there was no significant addition in secondary tiller number. Generally, the average mean number of tertiary tiller accross the accessions was far below compared to the number of primary and secondary tiller. The highest number of primary tiller, i.e. 8, was expressed by Pae Daye Indolobye (North Sulawesi), Padi Pulut Pute Iteung (East Kalimantan) and K. Puyuk (Central Kalimantan). Up to 22 secondary tillers were formed by Gadabung, a local upland rice variety from Central Kalimantan. Information on tillering ability of local upland variety will benefit rice breeder for selecting appropriate accessions as a gene source for breeding.
2020-01-01T00:00:00Z