Mekanisme Ketahanan Terinduksi oleh Plant Growth Promotting Rhizobacteria (PGPR) pada Tanaman Cabai Terinfeksi Cucumber Mosaik Virus (CMV)

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dc.contributor en-US
dc.creator Taufik, M
dc.creator Rahman, A
dc.creator Wahab, A
dc.creator Hidayat, S H
dc.date 2013-08-02
dc.date.accessioned 2018-05-02T06:25:28Z
dc.date.available 2018-05-02T06:25:28Z
dc.date.issued 2013-08-02
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/jhort/article/view/731
dc.identifier 10.21082/jhort.v20n3.2010.p%p
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/928
dc.description ABSTRAK. Plant growth promoting rhizobacteria (PGPR) dapat digunakan untuk menekan insiden penyakitmelalui mekanisme induksi ketahanan secara sistemik atau menghasilkan hormon tumbuh. Tujuan penelitian adalahmengetahui mekanisme ketahanan terinduksi pada tanaman cabai yang terinfeksi cucumber mosaik virus (CMV)dengan mengukur akumulasi asam salisilat (SA) dan peroksidase, deteksi CMV dengan teknik RT-PCR, dan analisispola protein tanaman. Hasil penelitian menunjukkan bahwa aplikasi PGPR dapat mereduksi insiden penyakit padatanaman yang terinfeksi CMV. Aplikasi PGPR secara signifikan dapat meningkatkan pertumbuhan tanaman cabaidan mengurangi insiden penyakit meskipun terinfeksi oleh CMV. Campuran isolat PG 01 dan BG 25 menghasilkanpertumbuhan tanaman yang lebih baik dibandingkan dengan perlakuan lainnya. Insiden penyakit yang ditunjukkanpada cabai yang diberi perlakuan campuran PG 01 dan BG 25 serta isolat BG 25 yang diberikan secara tunggalmencapai 8,33%, sementara insidensi penyakit pada cabai yang diberi isolat PG 01 secara tunggal mencapai 16,67%.Akumulasi SA dan peroksidase terjadi lebih tinggi pada tanaman cabai yang diaplikasi dengan PGPR. Teknik PCRmenggunakan primer spesifik berhasil mengamplifikasi genom CMV. Pola pita protein total tanaman yang diuji tidakberbeda dengan kontrol.ABSTRACT. Taufik, M., A. Rahman, A. Wahab, and S.H. Hidayat. 2010. Induced Resistant Mechanism byPlant Growth Promoting Rhizobacteria (PGPR) on Pepper Plants Infected By Cucumber Mosaic Virus (CMV).Plant growth promoting rhizobacteria can suppress disease intensity through systematical induction resistance or yieldhormone of growth regulator. The purpose of this research was to determine the mechanism of induced resistance CMVinfected chilli plants by measureing accumulate salicylated acid (SA), peroxidase (reverse transcriptase-polymerasechain reaction) (RT-PCR) and banding patterns of plant protein. The results showed that the application of PGPR canreduce of disease incidence pepper plants that was infected by CMV. Application PGPR can significantly improveplant growth and reduce the occurence of CMV infection symptoms. Mixture of isolates PG 01 and BG 25 gave betterresponse of plant growth compared to other treatments. The occurence of the disease on infected chilli plants treatedwith isolates mixture of PG 01 and BG 25, and single isolate of BG 25 was 8.33%, while the application of singleisolate of PG 01 caused the desease intensity up to 16.67%. The accumulation of salicylates acid and peroxydasewas higher in the chilli plants applied by PGPR. The technique of RT-PCR was able to detect CMV using spesificprimers. No difference of total protein band patterns between tested plants and control. en-US
dc.format application/pdf
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Center for Horticulture Research and Development en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/jhort/article/view/731/549
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/jhort/article/view/731/550
dc.rights Copyright (c) 2013 Indonesian Center for Horticulture Research and Development en-US
dc.rights http://creativecommons.org/licenses/by-sa/4.0 en-US
dc.source 2502-5120
dc.source 0853-7097
dc.source Jurnal Hortikultura; Vol 20, No 3 (2010): September 2010 en-US
dc.title Mekanisme Ketahanan Terinduksi oleh Plant Growth Promotting Rhizobacteria (PGPR) pada Tanaman Cabai Terinfeksi Cucumber Mosaik Virus (CMV) en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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