Karakterisasi Molekuler Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) Menggunakan Sekuen DNA Polimerase
No Thumbnail Available
Date
2021-09-15
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
KOMISI NASIONAL SUMBER DAYA GENETIK
Abstract
Corn borer (Helicoverpa armigera) is one of the most important pests that threaten corn production. H. armigera attacks cause corn production to decrease up to 80%. The use of the biological agent Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) is one of many ways to control the pest’s population along with the principles of Integrated Pest Management (IPM). This study aims to obtain the molecular characteristics of H. armigera NPV . NPV isolates used obtained from a collection of Insect Pathology Laboratory that were propagated in H. armigera larvae that were reared in the laboratory. Isolated DNA was amplified using the PCR method using forward primer (5'-GAT CGT TGA CCG ACG TCA ATA -3 ') and reverse primer (5'-CGA TGA CAC GTA GCC AGT AAA -3'), then homology and phylogenetics tests are carried out to analyze the relationship of HearNPV Bogor isolate with NPV isolates from other countries that have been registered on GeneBank. Based on the analysis of the nucleotide and amino acid sequences of DNA polymerase, the Bogor HearNPV isolate had a genetic relationship with NPV that infect H. armigera from Spain and India, H. assulta from China and H. zea from Brazil. Based on phylogenetic analysis the Bogor HearNPV is in the same group as NPV of H. armigera from Spain and India, H. assulta from China and H. zea from Brazil.
Description
Keywords
Biological agents, corn borer, DNA polymerase, molecular characterization