Mikroanalisis Kandungan Senyawa Fenolik Total Ekstrak Biji Kedelai dengan Reagen Folin-Ciocalteu

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One of the roles of phenolic compounds is as chemical defense system to inhibit the growth ofpathogen infection.Folin-Ciocalteu (FC) reagent is used to estimate total phenolic compounds spectrophotometrically. Microanalysis of the phenolic compounds using FC reagent is still limited.This study, therefore, aimed to develop a rapid method to estimate total phenolic compounds insoybean seed extract in a 96-well microplate. Optimum kinetic reaction was achieved after 90minutes of incubation. Response of 25 – 1.000 µg ml-1 of gallic acid was linear (r = 0.99). Hundredsof samples could be analysed using the microplate 2 format in limited time, such as when workingwith soybean germplasm collection.
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