Selection Resistance of Rice Germplasm Accessions to Bacterial Leaf Blight
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Date
2014
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Sekretariat Komisi Nasional Plasma Nutfah
Abstract
Rice cultivation in Indonesia has been faced with many obstacles, including the attack of bacterial leaf blight (BLB) that caused by bacteria Xanthomonas oryzae pv. oryzae (Xoo).
Resistant variety was considered as the cheap technique to control BLB disease and could be used by rice farmers. Germplasm accessions as a source to build resistant varieties must be known their resistance to BLB disease. This study aimed at selecting the resistance germplasm accessions to BLB pathotype III, IV, and VIII. The study was conducted at the Experimental Station of the Indonesian Center for Rice Research in Sukamandi, Subang, West Java at wet season 2012/2013 and dry season 2013 with Split Plot Design. The main plot was three pathotypes Xoo i.e. pathotype III, IV, and
VIII. The subplot was 20 germplasm accessions and three check varieties. A total of 20 hills of rice plants per plot were inoculated by Xoo with cutting method. Inoculation was
conducted before the primordia stage. Inoculation of bacterial suspension containing Xoo aged 48 hours at a concentration of 108 cfu. Observations BLB disease severity was done by measuring the longest length of symptoms at the age of two, three, and four weeks after inoculation (WAI). The result showed that three germplasm accessions from near isogenic lines were not significantly different with the check varieties (Angke) in their resistance to Xoo pathotype III, IV, and VIII in two cropping seasons. The isogenic lines were IRBB 55, IRBB 60, and IRBB 61 can be used in the assembly of resistant new varieties to BLB.
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Keywords
Selection of resistance, rice germplasm accessions, bacterial leaf blight.