Pengaruh Komposisi Media Dasar, Penambahan BAP, dan Pikloram terhadap Induksi Tunas Bawang Merah
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Indonesian Center for Horticulture Research and Development
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ABSTRAK. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Sayuran pada bulan Maret-Juni 2005, dengan tujuan untuk mengetahui pengaruh penambahan zat pengatur tumbuh BAP dan pikloram pada media dasar MS dan B5 terhadap pertumbuhan tunas bawang merah kultivar Sumenep. Bahan tanaman yang digunakan adalah jaringan meristematik sedangkan media tumbuh yang diuji adalah media dasar MS dan B5 dengan konsentrasi BAP (0, 1, dan 2 mg/l), pikloram (0, 0,1, dan 0,2 mg/l). Hasil penelitian menunjukkan bahwa ada perbedaan pertumbuhan plantlet akibat penambahan zat pengatur tumbuh BAP dan pikloram. Persentase daun normal terbesar didapat dari perlakuan B5 tanpa pikloram dan BAP, media MS tanpa pikloram. Pertumbuhan akar plantlet lebih baik yang dikulturkan pada media MS tanpa hormon atau dengan penambahan hormon dengan konsentrasi terendah.ABSTRACT. Karjadi, A.K. and Buchory, A. 2008. The Effect of Additional BAP and Picloram to Basal Medium MS and B5 on Shoot Induction of Shallot. The`experiment was conducted in Tissue Culture Laboratory of Indonesian Vegetable Research Institute in Lembang on March-June 2005. Objective of the experiment was to find out the effect of additional BAP and picloram to basal medium MS and B5 on shoot induction of shallot cv. Sumenep. The treatments were culturing meristem tissue (shoot tip) on medium MS and B5 with concentration of BAP (0, 1, and 2 mg/l), and picloram (0, 0,1, and 0,2 mg/l). The results showed that there were differences on plantlet growth with addition of BAP and picloram. Meristematic tissue of shallot cv. Sumenep could be proliferated on medium MS or B5. The highest percentage of normal leaf growth was obtained by B5 medium without picloram or BAP, and MS medium without picloram. The best roots growth was found in culture of MS medium without or with low concentration of hormone.
Keywords
Allium ascalonicum; MS; B5; Pikloram; BAP; Jaringan meristematik; Pertumbuhan tunas