Effect of N-acetylcystein on ERK Gene Expression in Ovarian Tissue of Acrylamide-Treated Adult Rats

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dc.contributor en-US
dc.creator Naimi, Marziyeh; Department of Biology, Fars Science and Research Branch, Islamic Azad University, Fars; Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
dc.creator Shariati, Mehrdad; Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun
dc.creator Naeimi, Sirous; Department of Genetics, Kazerun Branch, Islamic Azad University, Kazerun, Iran
dc.creator Edalatmanesh, Mohammad Amin; Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
dc.date 2020-03-17
dc.date.accessioned 2020-11-06T02:28:22Z
dc.date.available 2020-11-06T02:28:22Z
dc.identifier http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/2161
dc.identifier 10.14334/jitv.v25i1.2161
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/10423
dc.description Acrylamide (AA) is a toxic and carcinogenic compound produced in cooking process. The purpose of this study is to evaluate the effect of N-acetylcysteine (NAC) on extracellular signal-regulated kinase (ERK) gene expression level and ovarian histopathological changes in AA-treated rats. Thirty-six female adult Wistar rats were randomly divided into 6 groups including control, positive control (+VE Con), negative control (-VE Con), experimental 1 (Exp1), experimental 2 (Exp2) and experimental 3 (Exp3). Twenty eight days after the treatment, ERK gene expression level was measured by real-time PCR method and ovarian histopathological changes were evaluated. The ERK gene expression level was significantly decreased in the +VE Con, Exp1 and Exp2 groups as compared to the control group (p˂0.05), but not in the -VE Con and Exp3 groups (p˃0.05). Histologically, the +VE Con group showed a significant decrease in the number of primary, secondary and Graafian follicles as well as corpus luteum as compared to the control group (p˂0.05), but not in the negative, Exp2 and Exp3 groups (p˃0.05). In the Exp1 group, the number of primary and secondary follicles as well as corpus luteum significantly decreased (p˂0.05), however, the numbers of Graafian follicle and the corpus luteum were significantly increased as compared to the +VE Con group (p˂0.05). The AA was supposed to increase the apoptosis and folliculogenesis degradation in the rat ovarian tissue by decreasing ERK gene expression. Administration of NAC ameliorated the deleterious effects of AA in a dose-dependent manner and improve folliculogenesis by reducing apoptosis level. Thus, the NAC supplement could be helpful in ameliorating animal fertility. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Center for Animal Research and Development (ICARD) en-US
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/2161/2029
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/downloadSuppFile/2161/149
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/downloadSuppFile/2161/150
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/downloadSuppFile/2161/151
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/downloadSuppFile/2161/152
dc.relation http://medpub.litbang.pertanian.go.id/index.php/jitv/article/downloadSuppFile/2161/153
dc.rights Copyright (c) 2020 Indonesian Journal of Animal and Veterinary Sciences en-US
dc.source Jurnal Ilmu Ternak dan Veteriner; Vol 25, No 1 (2020): MARCH 2020; 11-18 en-US
dc.source 2252-696X
dc.source 0853-7380
dc.subject Acrylamide, Apoptosis, ERK, Female Rat, N-acetylcysteine en-US
dc.title Effect of N-acetylcystein on ERK Gene Expression in Ovarian Tissue of Acrylamide-Treated Adult Rats en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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